pcDNA3-AU1-mTOR-E2419K
(Plasmid #19994)

• Depositing Lab: Fuyuhiko Tamanoi
• Publication: Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mammalian target of rapamycin-E2419K
• Alt name: mTOR-E2419K
• Species: R. norvegicus (rat)
• Insert Size (bp): 7700
• Mutation: Changed Glutamine 2419 to Lysine
• Entrez Gene: Mtor (a.k.a. Frap1, RAFT1)
• Tag / Fusion Protein:
  • AU1 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry