pGBT9-NF1-GRD
(Plasmid #19993)

• Depositing Lab: Fuyuhiko Tamanoi
• Publication: Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503.

Backbone

• Vector backbone: pGBT9
• Backbone manufacturer: clonetech
• Backbone size w/o insert (bp): 5524
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Neurofibromatosis type I
• Alt name: NF1
• Alt name: NF1-GRD
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Entrez Gene: NF1 (a.k.a. NFNS, VRNF, WSS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: kpnI (not destroyed)
• 3′ cloning site: PvuII (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The authors note that there is a mutation in the coding sequence: C3918T

"This created a T to I change within the GAP domain of NF1. However, this
change is outside of the conserved blocks within the GAP domain. This
particular residue (1195 of NF1-GRD shown in figure 4 in the chapter by
M.R. Kim and F. Tamanoi, chapter 6 in Neurofibromatosis type I from
genotype to phenotype, editor by M. Upadhyaya and D. N. Cooper BIOS
scientific publisher) is not conserved among different GAP proteins.
Therefore, we believe that the amino acid change does not affect the GAP
activity. In fact, we have used this construct for the wild-type GAP
activity."