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Addgene

pcihygrosynDAT
(Plasmid #19992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcihygro
  • Backbone size w/o insert (bp) 5764
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synDAT
  • Alt name
    SLC6A3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7624
  • Entrez Gene
    SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site kpni (not destroyed)
  • 3′ cloning site noti (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcihygrosynDAT was a gift from Jonathan Javitch (Addgene plasmid # 19992 ; http://n2t.net/addgene:19992 ; RRID:Addgene_19992)

  • For your References section:

    Amphetamine-induced loss of human dopamine transporter activity: an internalization-dependent and cocaine-sensitive mechanism. Saunders C, Ferrer JV, Shi L, Chen J, Merrill G, Lamb ME, Leeb-Lundberg LM, Carvelli L, Javitch JA, Galli A. Proc Natl Acad Sci U S A. 2000 Jun 6. 97(12):6850-5. 10.1073/pnas.110035297 PubMed 10823899
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