yfpsyndat
(Plasmid
#19991)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcihygro
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 6516
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyfpsynhdat
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Alt nameSLC6A3
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SpeciesH. sapiens (human)
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Insert Size (bp)8376
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Entrez GeneSLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
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Tag
/ Fusion Protein
- yfp (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site bsrgi (not destroyed)
- 3′ cloning site noti (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
yfpsyndat was a gift from Jonathan Javitch (Addgene plasmid # 19991 ; http://n2t.net/addgene:19991 ; RRID:Addgene_19991) -
For your References section:
PI 3-kinase regulation of dopamine uptake. Carvelli L, Morón JA, Kahlig KM, Ferrer JV, Sen N, Lechleiter JD, Leeb-Lundberg LM, Merrill G, Lafer EM, Ballou LM, Shippenberg TS, Javitch JA, Lin RZ, Galli A. J Neurochem. 2002 May . 81(4):859-69. 10.1046/j.1471-4159.2002.00892.x PubMed 12065645
