pcihygroflagsynhDAT
(Plasmid #19990)

• Depositing Lab: Jonathan Javitch
• Publication: Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97(12):6850-5.

Backbone

• Vector backbone: pcin4, pIREShygro
• Backbone manufacturer: Glaxowellcome,CLONTECH.
• Backbone size w/o insert (bp): 5764
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dopamine transporter
• Alt name: SLC6A3
• Species: H. sapiens (human)
• Insert Size (bp): 7683
• Entrez Gene: SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: bsrgi (not destroyed)
• 3′ cloning site: noti (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry