E1_pENTR_L1-L4_NN_G1333-DddA11'-C
(Plasmid
#199800)
-
PurposeptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 199800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePINII
-
Vector typeTALEN
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor
-
SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Multisite Gateway entry vector to express one hand of pTALECD_v2-UGI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E1_pENTR_L1-L4_NN_G1333-DddA11'-C was a gift from Shin-ichi Arimura (Addgene plasmid # 199800 ; http://n2t.net/addgene:199800 ; RRID:Addgene_199800) -
For your References section:
Characterization and development of a plastid genome base editor, ptpTALECD. Nakazato I, Okuno M, Itoh T, Tsutsumi N, Arimura SI. Plant J. 2023 Aug;115(4):1151-1162. doi: 10.1111/tpj.16311. Epub 2023 Jun 2. 10.1111/tpj.16311 PubMed 37265080