Skip to main content
Addgene

hPU.1ΔN165-pET28b
(Plasmid #199796)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Induce with 0.5 mM IPTG at 30°C for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    spi1
  • Alt name
    Spi-1 proto-oncogene
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    326
  • Entrez Gene
    SPI1 (a.k.a. AGM10, OF, PU.1, SFPI1, SPI-1, SPI-A)
  • Tag / Fusion Protein
    • None (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7promoter
  • 3′ sequencing primer T7terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPU.1ΔN165-pET28b was a gift from Gregory Poon (Addgene plasmid # 199796 ; http://n2t.net/addgene:199796 ; RRID:Addgene_199796)

  • For your References section:

    Intrinsic disorder controls two functionally distinct dimers of the master transcription factor PU.1. Xhani S, Lee S, Kim HM, Wang S, Esaki S, Ha VLT, Khanezarrin M, Fernandez GL, Albrecht AV, Aramini JM, Germann MW, Poon GMK. Sci Adv. 2020 Feb 21;6(8):eaay3178. doi: 10.1126/sciadv.aay3178. eCollection 2020 Feb. 10.1126/sciadv.aay3178 PubMed 32128405
Related items: