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Addgene

pCMV5-Fyn C3,6S
(Plasmid #19979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4657
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fyn
  • Alt name
    Src kinase p59
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Mutation
    C3,6S
  • Entrez Gene
    FYN (a.k.a. SLK, SYN, p59-FYN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-Fyn C3,6S was a gift from Marilyn Resh (Addgene plasmid # 19979 ; http://n2t.net/addgene:19979 ; RRID:Addgene_19979)

  • For your References section:

    Dual myristylation and palmitylation of Src family member p59fyn affects subcellular localization. Alland L, Peseckis SM, Atherton RE, Berthiaume L, Resh MD. J Biol Chem. 1994 Jun 17. 269(24):16701-5. PubMed 8206991
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