pET-Duet1-His-TEV-mCherry-p62
(Plasmid #199780)

• Purpose: Used for the expression of mCherry labelled p62 (SMC Internal No. 391).
• Depositing Lab: Sascha Martens
• Publication: Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.

Backbone

• Vector backbone: pET-Duet1
• Backbone size w/o insert (bp): 5386
• Total vector size (bp): 7463
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p62
• Alt name: NUP62
• Species: H. sapiens (human)
• Insert Size (bp): 2077
• GenBank ID: CAA41411.1
• Entrez Gene: NUP62 (a.k.a. IBSN, SNDI, p62)
• Tag / Fusion Protein:
  • 6x His, TEV, mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: Not1 (unknown if destroyed)
• 5′ sequencing primer: ATGCGTCCGGCGTAGA
• 3′ sequencing primer: TGCggccGCTCACAACG

Resource Information

• Supplemental Documents:
  • SMC391 - pETDuet-mCherry-p62.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry