pET28a-cMSP1E3D1
(Plasmid #199772)

• Purpose: Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)
• Depositing Lab: Franz Hagn
• Publication: Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861. doi: 10.1016/j.jmb.2022.167861. Epub 2022 Oct 20.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6656
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cMSP1E3D1
• Alt name: Circular Membrane Scaffold Protein 1E3D1
• Species: Synthetic
• Insert Size (bp): 1287
• Mutation: Extended cMSP1D1; external His-Tags flanking the C and N termini
• Tags / Fusion Proteins:
  • His6-Tag (N terminal on insert)
  • His6-Tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 Promotor
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry