pET28a-cMSP1D1
(Plasmid #199768)

• Purpose: Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)
• Depositing Lab: Franz Hagn
• Publication: Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-1933. doi: 10.1002/cbic.201800345. Epub 2018 Jul 30.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6458
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cMSP1D1
• Alt name: Circular Membrane Scaffold Protein 1D1
• Species: Synthetic
• Insert Size (bp): 1089
• Mutation: external His-Tags flanking the C and N termini
• Tags / Fusion Proteins:
  • His6-Tag (N terminal on insert)
  • His6-Tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 Promotor
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry