pET28a-cMSPdH4-6
(Plasmid
#199766)
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PurposeExpresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199766 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6197
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecMSPdH46
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Alt nameCircular Membrane Scaffold Protein Helix 4-6 deletion
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SpeciesSynthetic
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Insert Size (bp)828
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Mutationinternal His-Tag
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Tag
/ Fusion Protein
- His6-Tag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-cMSPdH4-6 was a gift from Franz Hagn (Addgene plasmid # 199766 ; http://n2t.net/addgene:199766 ; RRID:Addgene_199766) -
For your References section:
A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins. Miehling J, Goricanec D, Hagn F. Chembiochem. 2018 Sep 17;19(18):1927-1933. doi: 10.1002/cbic.201800345. Epub 2018 Jul 30. 10.1002/cbic.201800345 PubMed 29947468
