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Addgene

PGK-EGFP-FKBPF36V
(Plasmid #199765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1-hPGK-Blast
  • Backbone size w/o insert (bp) 4814
  • Total vector size (bp) 5936
  • Vector type
    Mammalian Expression, Worm Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-dTAG
  • Insert Size (bp)
    1089
  • Promoter hPGK
  • Tag / Fusion Protein
    • GFP and dTAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer gagaggtcggtgattcggtc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGK-EGFP-FKBPF36V was a gift from Hannes Lans (Addgene plasmid # 199765 ; http://n2t.net/addgene:199765 ; RRID:Addgene_199765)

  • For your References section:

    Recovery of protein synthesis to assay DNA repair activity in transcribed genes in living cells and tissues. van der Woude M, Davo-Martinez C, Thijssen KL, Vermeulen W, Lans H. Nucleic Acids Res. 2023 Oct 13;51(18):e93. doi: 10.1093/nar/gkad642. 10.1093/nar/gkad642 PubMed 37522336
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