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TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
(Plasmid #199763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Backbone size w/o insert (bp) 5449
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LacZ-nLucPEST
  • Species
    E.coli
  • Insert Size (bp)
    3072
  • Promoter TetO7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    nanoLuc source: Anna et al. (https://doi.org/10.1002/yea.3155)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4) was a gift from Brian Zid (Addgene plasmid # 199763 ; http://n2t.net/addgene:199763 ; RRID:Addgene_199763)

  • For your References section:

    Differential translation elongation directs protein synthesis in response to acute glucose deprivation in yeast. Guzikowski AR, Harvey AT, Zhang J, Zhu S, Begovich K, Cohn MH, Wilhelm JE, Zid BM. RNA Biol. 2022;19(1):636-649. doi: 10.1080/15476286.2022.2065784. Epub 2021 Dec 31. 10.1080/15476286.2022.2065784 PubMed 35491906
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