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pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
(Plasmid #199759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG306
  • Backbone manufacturer
    Susan Lindquist
  • Backbone size w/o insert (bp) 6656
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    synYFP[TTG/AGA]-4xArg[CGA]-nLuc
  • Alt name
    optYFP-4_CGA
  • Species
    Synthetic
  • Insert Size (bp)
    726
  • Promoter TetO7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    nanoLuc source: Anna et al. (https://doi.org/10.1002/yea.3155), synYFP[TTG] source: Subramaniam Lab

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc was a gift from Brian Zid (Addgene plasmid # 199759 ; http://n2t.net/addgene:199759 ; RRID:Addgene_199759)

  • For your References section:

    Quantification of elongation stalls and impact on gene expression in yeast. Hou W, Harjono V, Harvey AT, Subramaniam AR, Zid BM. bioRxiv. 2023 Mar 20:2023.03.19.533377. doi: 10.1101/2023.03.19.533377. Preprint. 10.1101/2023.03.19.533377 PubMed 36993688
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