TccCas13a Twin SUMO
(Plasmid #199754)

• Purpose: Expresses codon optimized thermostabile TccCas13a in E.coli
• Depositing Lab: Magdy Mahfouz
• Publication: Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2118260119. doi: 10.1073/pnas.2118260119. Epub 2022 Jun 28.

Backbone

• Vector backbone: Twin-SUMO
• Backbone size w/o insert (bp): 6076
• Total vector size (bp): 9761
• Vector type: Bacterial Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TccCas13a
• Species: Thermoclostridium caenicola
• Insert Size (bp): 3678
• Promoter: LacI
• Tag / Fusion Protein:
  • SUMO (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: gaaattaatacgactcactatagggg
• 3′ sequencing primer: caaaaaacccctcaagacccg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry