pCBLsgRNA5
(Plasmid
#199731)
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PurposeGolden Gate entry vector; 5th gRNA under ZmU6-C3 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYPQ131D
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameZmU6C3-sgRNA5 scaffold
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SpeciesSynthetic
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Insert Size (bp)327
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCBLsgRNA5 was a gift from Kan Wang (Addgene plasmid # 199731 ; http://n2t.net/addgene:199731 ; RRID:Addgene_199731) -
For your References section:
New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis. Lee K, Kang M, Ji Q, Grosic S, Wang K. Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231. 10.1093/plphys/kiad231 PubMed 37070560