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Addgene

pCBL101.1
(Plasmid #199724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCBL101
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Multiple Cloning Site, NPTII
  • Species
    Synthetic
  • Insert Size (bp)
    50
  • Mutation
    "GAGACC" to "GATACC"

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCBL101.1 was a gift from Kan Wang (Addgene plasmid # 199724 ; http://n2t.net/addgene:199724 ; RRID:Addgene_199724)

  • For your References section:

    New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis. Lee K, Kang M, Ji Q, Grosic S, Wang K. Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231. 10.1093/plphys/kiad231 PubMed 37070560
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