pCBL101
(Plasmid
#199722)
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PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTF101.1
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNPTII
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Alt nameneomycin phosphotransferase II
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SpeciesSynthetic
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Insert Size (bp)3209
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCBL101 was a gift from Kan Wang (Addgene plasmid # 199722 ; http://n2t.net/addgene:199722 ; RRID:Addgene_199722) -
For your References section:
New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis. Lee K, Kang M, Ji Q, Grosic S, Wang K. Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231. 10.1093/plphys/kiad231 PubMed 37070560
