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Addgene

pKL2351
(Plasmid #199721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ210
  • Backbone manufacturer
    Yiping Qi
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NPTII
  • Alt name
    neomycin phosphotransferase II
  • Species
    Synthetic
  • Insert Size (bp)
    3209

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A 185T mutation was found in mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL2351 was a gift from Kan Wang (Addgene plasmid # 199721 ; http://n2t.net/addgene:199721 ; RRID:Addgene_199721)

  • For your References section:

    New T-DNA binary vectors with NptII selection and RUBY reporter for efficient maize transformation and targeted mutagenesis. Lee K, Kang M, Ji Q, Grosic S, Wang K. Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231. 10.1093/plphys/kiad231 PubMed 37070560
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