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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19972 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 5270
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRpl26
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Alt nameribosomal protein L26, SIG-20
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SpeciesH. sapiens (human)
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Insert Size (bp)484
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Entrez GeneRPL26 (a.k.a. DBA11, L26)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Made by Yaara Ofir-Rosenfeld
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-L26-Flag was a gift from Moshe Oren (Addgene plasmid # 19972 ; http://n2t.net/addgene:19972 ; RRID:Addgene_19972) -
For your References section:
Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26. Ofir-Rosenfeld Y, Boggs K, Michael D, Kastan MB, Oren M. Mol Cell. 2008 Oct 24. 32(2):180-9. 10.1016/j.molcel.2008.08.031 PubMed 18951086
Map uploaded by the depositor.
