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Addgene

pEGFP-TdIF2
(Plasmid #199699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TdIF2
  • Alt name
    ERBP
  • Alt name
    AB_046574
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer EGFP side
  • 3′ sequencing primer SV40 Poly A side
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Obtained by screening the human cDNA library (purchased from Origene).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-TdIF2 was a gift from Kenji Sugimoto (Addgene plasmid # 199699 ; http://n2t.net/addgene:199699 ; RRID:Addgene_199699)

  • For your References section:

    Characterization of nucleolar localization and exclusion signals in terminal deoxynucleotidyltransferase interacting factor 2/estrogen receptor alpha-binding protein. Fukada T, Shibata S, Ueda T, Sasaki K, Shimoida Y, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2019 Jul;83(7):1255-1262. doi: 10.1080/09168451.2019.1591265. Epub 2019 Mar 24. 10.1080/09168451.2019.1591265 PubMed 30907250
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