pKeima-LiveDrop
(Plasmid #199695)

• Purpose: lipophagy reporter system based on the Keima-fluorophore
• Depositing Lab: Tobias Walther
• Publication: Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110. doi: 10.1038/s41556-023-01178-w. Epub 2023 Jul 13.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 4909
• Modifications to backbone: +Keima-LiveDrop
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GPAT4
• Alt name: AGPAT6; LPAATZ; TSARG7; 1-AGPAT 6; LPAAT-zeta
• Species: H. sapiens (human)
• Insert Size (bp): 171
• Entrez Gene: GPAT4 (a.k.a. 1-AGPAT 6, AGPAT6, LPAAT-zeta, LPAATZ, TSARG7)
• Promoter: CMV
• Tag / Fusion Protein:
  • Keima (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI, BmtI (unknown if destroyed)
• 3′ cloning site: PspXI, PaeR7I, XhoI (unknown if destroyed)
• 5′ sequencing primer: GCACAGCTAGCGCCACCATGGTGAGCGTGATCGCC
• 3′ sequencing primer: GCACACTCGAGCGCCCAGCAGGGAGTGGCGGGCGATG

Resource Information

• Supplemental Documents:
  • pKeima-fLiveDrop[87].gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry