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pCAG-1700029I15Rik-3×FLAG
(Plasmid #199689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG1.1
  • Backbone manufacturer
    Ikawa Lab
  • Backbone size w/o insert (bp) 5259
  • Total vector size (bp) 5556
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1700029I15Rik
  • Alt name
    Frey
  • Alt name
    Frey1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    297
  • Entrez Gene
    1700029I15Rik
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GCCTTCTTCTTTTTCCTACAGC
  • 3′ sequencing primer GCCACACCAGCCACCACCTTCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-1700029I15Rik-3×FLAG was a gift from Masahito Ikawa (Addgene plasmid # 199689 ; http://n2t.net/addgene:199689 ; RRID:Addgene_199689)

  • For your References section:

    1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Lu Y, Shimada K, Tang S, Zhang J, Ogawa Y, Noda T, Shibuya H, Ikawa M. Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2207263120. doi: 10.1073/pnas.2207263120. Epub 2023 Feb 14. 10.1073/pnas.2207263120 PubMed 36787362
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