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pFB-CG-LYCHOS FP>IA-HA-GFP
(Plasmid #199688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastbac
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LYCHOS
  • Alt name
    GPR155
  • Species
    H. sapiens (human)
  • Mutation
    F43 and P44 mutated to I and A respectively
  • Entrez Gene
    GPR155 (a.k.a. DEP.7, DEPDC3, PGR22)
  • Tags / Fusion Proteins
    • EGFP (C terminal on insert)
    • HA (C terminal on insert)
    • 6xHis (C terminal on insert)
    • HRV 3C (C terminal on insert)
    • 10xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BstBI (not destroyed)
  • 5′ sequencing primer aaatgataaccatctcgc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB-CG-LYCHOS FP>IA-HA-GFP was a gift from Roberto Zoncu (Addgene plasmid # 199688 ; http://n2t.net/addgene:199688 ; RRID:Addgene_199688)

  • For your References section:

    Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency to mTORC1. Shin HR, Citron YR, Wang L, Tribouillard L, Goul CS, Stipp R, Sugasawa Y, Jain A, Samson N, Lim CY, Davis OB, Castaneda-Carpio D, Qian M, Nomura DK, Perera RM, Park E, Covey DF, Laplante M, Evers AS, Zoncu R. Science. 2022 Sep 16;377(6612):1290-1298. doi: 10.1126/science.abg6621. Epub 2022 Aug 25. 10.1126/science.abg6621 PubMed 36007018
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