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pcDNA-D2s-L-V1
(Plasmid #19967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D2 short variant-N terminal Venus
  • Alt name
    D2R; D2DR; DRD2
  • Species
    H. sapiens (human); Aequorea Victoria
  • Insert Size (bp)
    1857
  • Entrez Gene
    DRD2 (a.k.a. D2DR, D2R)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • N terminal Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-D2s-L-V1 was a gift from Jonathan Javitch (Addgene plasmid # 19967 ; http://n2t.net/addgene:19967 ; RRID:Addgene_19967)

  • For your References section:

    Dopamine D2 receptors form higher order oligomers at physiological expression levels. Guo W, Urizar E, Kralikova M, Mobarec JC, Shi L, Filizola M, Javitch JA. EMBO J. 2008 Sep 3. 27(17):2293-304. 10.1038/emboj.2008.153 PubMed 18668123

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