BAF-S4D-tet-puro
(Plasmid
#199650)
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PurposeDoxycycline-inducible BAF expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLXI403
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBAF
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SpeciesH. sapiens (human)
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MutationS4D
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Entrez GeneBANF1 (a.k.a. BAF, BCRP1, D14S1460, NGPS)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BAF-S4D-tet-puro was a gift from William Hahn (Addgene plasmid # 199650 ; http://n2t.net/addgene:199650 ; RRID:Addgene_199650) -
For your References section:
VRK1 as a synthetic lethal target in VRK2 promoter-methylated cancers of the nervous system. So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC, Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, Stegmaier K, Filbin MG, Hahn WC. JCI Insight. 2022 Oct 10;7(19):e158755. doi: 10.1172/jci.insight.158755. 10.1172/jci.insight.158755 PubMed 36040810