pJH1371
(Plasmid #199631)

• Purpose: Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP
• Depositing Lab: Mei Zhen
• Publication: Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. Epub 2024 Apr 10.

Backbone

• Vector backbone: pBSK
• Backbone size w/o insert (bp): 8542
• Total vector size (bp): 8622
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GPI
• Insert Size (bp): 80
• Promoter: Pregf-1
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGGAGGACCTTCGACCTCGAGGGGGGGCCCggtaccggtggataatgaaaacaatcat
• 3′ sequencing primer: AAAAGTTCTTCTCCTTTACTCATatcgatgccaggcgggtcttgtgcgacgac

Resource Information

• Supplemental Documents:
  • pJH1371

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.

Please note: This plasmid contains a single nucleotide mutation in YFP second half and has CD58 and C. elegans strain Bristol N2 chromosome II sequences instead of UNC-54 3'UTR. These differences are not known to affect plasmid function.