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CAG_mCh_p85a_mCh
(Plasmid #199630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry_C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p85a-SH2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    339
  • Entrez Gene
    Pik3r1 (a.k.a. PI3K, p50alpha, p55alpha, p85alpha)
  • Promoter CAG
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Common sequencing primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG_mCh_p85a_mCh was a gift from Ryohei Yasuda (Addgene plasmid # 199630 ; http://n2t.net/addgene:199630 ; RRID:Addgene_199630)

  • For your References section:

    Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors. Tu X, Jain A, Parra Bueno P, Decker H, Liu X, Yasuda R. Sci Adv. 2023 Aug 2;9(31):eadg0666. doi: 10.1126/sciadv.adg0666. Epub 2023 Aug 2. 10.1126/sciadv.adg0666 PubMed 37531435
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