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Addgene

CAG_mIGF1R_EGFP
(Plasmid #199629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CAG-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mIGF1R
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4104
  • Mutation
    Y1316F
  • Entrez Gene
    IGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)
  • Promoter CAG
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Common sequencing primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG_mIGF1R_EGFP was a gift from Ryohei Yasuda (Addgene plasmid # 199629 ; http://n2t.net/addgene:199629 ; RRID:Addgene_199629)

  • For your References section:

    Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors. Tu X, Jain A, Parra Bueno P, Decker H, Liu X, Yasuda R. Sci Adv. 2023 Aug 2;9(31):eadg0666. doi: 10.1126/sciadv.adg0666. Epub 2023 Aug 2. 10.1126/sciadv.adg0666 PubMed 37531435
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