pRep
(Plasmid
#199599)
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PurposeExpresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRep was a gift from George Church (Addgene plasmid # 199599 ; http://n2t.net/addgene:199599 ; RRID:Addgene_199599) -
For your References section:
Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design. Ogden PJ, Kelsic ED, Sinai S, Church GM. Science. 2019 Nov 29;366(6469):1139-1143. doi: 10.1126/science.aaw2900. 10.1126/science.aaw2900 PubMed 31780559