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Addgene

pLV-SARS-COV-2-5UTR-GLuc
(Plasmid #199592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 9056
  • Total vector size (bp) 9906
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 5UTR-GLuc
  • Alt name
    SARS-CoV-2
  • Insert Size (bp)
    849
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer aatgtagtcttatgcaatactctt
  • 3′ sequencing primer aagcttgcagctccagcttttgtt
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-SARS-COV-2-5UTR-GLuc was a gift from Jingxin Wang (Addgene plasmid # 199592 ; http://n2t.net/addgene:199592 ; RRID:Addgene_199592)

  • For your References section:

    Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5' untranslated region. Tang Z, Hegde S, Hao S, Selvaraju M, Qiu J, Wang J. Nat Commun. 2025 Jan 8;16(1):483. doi: 10.1038/s41467-024-55608-w. 10.1038/s41467-024-55608-w PubMed 39779694
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