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pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
(Plasmid #199585)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7m
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 10585
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
  • Alt name
    ERK tKiMBImut
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2112
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggtttagtgaaccgtcagatccgc
  • 3′ sequencing primer GGAAGAAAACCCAGGGCCT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    AkaLuc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1650
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAGTGGATAAAGCAAAGCTGTC
  • 3′ sequencing primer ttcttgagacaaaggcttgg
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Blasticidin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    395
  • Promoter CMV

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gacggcaagatcgccgtg
  • 3′ sequencing primer tcaagcttatcgataatcaacctctgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin was a gift from Michael Lin (Addgene plasmid # 199585 ; http://n2t.net/addgene:199585 ; RRID:Addgene_199585)

  • For your References section:

    Kinase-Modulated Bioluminescent Indicators Enable Noninvasive Imaging of Drug Activity in the Brain. Wu Y, Walker JR, Westberg M, Ning L, Monje M, Kirkland TA, Lin MZ, Su Y. ACS Cent Sci. 2023 Mar 20;9(4):719-732. doi: 10.1021/acscentsci.3c00074. eCollection 2023 Apr 26. 10.1021/acscentsci.3c00074 PubMed 37122464
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