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pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
(Plasmid #199582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX552
  • Backbone size w/o insert (bp) 5440
  • Total vector size (bp) 7140
  • Vector type
    Mammalian Expression, AAV, Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Chronos eGFP
  • Insert Size (bp)
    1700
  • GenBank ID
    KF992040
  • Promoter EF1a intron A
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCAAGTTAAAATAAGGC
  • 3′ sequencing primer GGCAACTTCCAGGGCCAGG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pAAV-EF1α1.1-FLPX-rc [Chronos-GFP] was a gift from Edward Boyden (Addgene plasmid # 122102 ; http://n2t.net/addgene:122102 ; RRID:Addgene_122102)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pX552 was a gift from Feng Zhang (Addgene plasmid # 60958 ; http://n2t.net/addgene:60958 ; RRID:Addgene_60958)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold) was a gift from Bryan Copits (Addgene plasmid # 199582 ; http://n2t.net/addgene:199582 ; RRID:Addgene_199582)

  • For your References section:

    Cell-Specific Single Viral Vector CRISPR/Cas9 Editing and Genetically Encoded Tool Delivery in the Central and Peripheral Nervous Systems. Moffa JC, Bland IN, Tooley JR, Kalyanaraman V, Heitmeier M, Creed MC, Copits BA. eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.2024. doi: 10.1523/ENEURO.0438-23.2024. Print 2024 Jul. 10.1523/ENEURO.0438-23.2024 PubMed 38871457
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