DDX5 D248N V5
(Plasmid #199577)

• Purpose: Expresses helicase dead DDX5 cloned into pLenti puro
• Depositing Lab: Michael Kastan
• Publication: McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1.

Backbone

• Vector backbone: pLenti puro
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7066
• Total vector size (bp): 8900
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX5
• Species: H. sapiens (human)
• Insert Size (bp): 1845
• Mutation: D248N
• GenBank ID: NM_001320595
• Entrez Gene: DDX5 (a.k.a. G17P1, HLR1, HUMP68, p68)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene # 88874

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry