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Addgene

FLAG SMG1 D2335A/N2340K
(Plasmid #199575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 16500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMG1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    11000
  • Mutation
    D2335A and N2340K
  • GenBank ID
    NM_015092
  • Entrez Gene
    SMG1 (a.k.a. 61E3.4, ATX, LIP)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG SMG1 D2335A/N2340K was a gift from Michael Kastan (Addgene plasmid # 199575 ; http://n2t.net/addgene:199575 ; RRID:Addgene_199575)

  • For your References section:

    Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced Alternative Splicing Pathway. McCann JJ, Fleenor DE, Chen J, Lai CH, Bass TE, Kastan MB. Radiat Res. 2023 Apr 1;199(4):406-421. doi: 10.1667/RADE-22-00219.1. 10.1667/RADE-22-00219.1 PubMed 36921295
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