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Addgene

pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)
(Plasmid #199566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroX-Tight-Pur
  • Backbone manufacturer
    Clontech
  • Vector type
    Retroviral ; Allows for Puromycin selection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trim69 Full length, isoform A (codon optimized)
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized
  • Entrez Gene
    TRIM69 (a.k.a. HSD-34, HSD34, RNF36, Trif)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized) was a gift from Andrea Cimarelli & Yuxin Song (Addgene plasmid # 199566 ; http://n2t.net/addgene:199566 ; RRID:Addgene_199566)

  • For your References section:

    Trim69 is a microtubule regulator that acts as a pantropic viral inhibitor. Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, Cimarelli A. Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 10.1073/pnas.2211467119. Epub 2022 Oct 17. 10.1073/pnas.2211467119 PubMed 36251989
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