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Addgene

pPfetFn-Target_v2
(Plasmid #199564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGG2121
  • Backbone size w/o insert (bp) 1500
  • Total vector size (bp) 6365
  • Modifications to backbone
    Based on pMTL82121, change teminator TCD0164 to TtyrS and add BsaI Golden-Gate sites
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SacB
  • gRNA/shRNA sequence
    AATTTCTACTGTTGTAGATtGAGACGattCGTCTCcAATTTCTACTGTTGTAGAT
  • Species
    Bacillus subtilis
  • GenBank ID
    936413
  • Promoter Pthl_fU

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer GCcgatcgTTTTTAACAAAATATATTGATAAAAATAATAATAGTGGGT
  • 3′ sequencing primer cgctgcagttatttgttaactgttaattgtccttg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPfetFn-Target_v2 was a gift from Philippe Lambin (Addgene plasmid # 199564 ; http://n2t.net/addgene:199564 ; RRID:Addgene_199564)

  • For your References section:

    Development of a CRISPR-Cas12a system for efficient genome engineering in clostridia. Zhang Y, Kubiak AM, Bailey TS, Claessen L, Hittmeyer P, Dubois L, Theys J, Lambin P. Microbiol Spectr. 2023 Nov 10:e0245923. doi: 10.1128/spectrum.02459-23. 10.1128/spectrum.02459-23 PubMed 37947521
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