pPIPL12-FnCas12a
(Plasmid #199563)

• Purpose: Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia
• Depositing Lab: Philippe Lambin
• Publication: Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923. doi: 10.1128/spectrum.02459-23.

Backbone

• Vector backbone: pGG3221
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 8909
• Modifications to backbone: Based on vectors pGG2121 and pMTL83221
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology ; Tetracycline Inducible

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 300 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For use in C. sporogenes and C. butyricum, use 15-20 μg/mL Erythromycin
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Fncas12a
• Alt name: Fncpf1
• Species: Francisella novicida
• Insert Size (bp): 4750
• GenBank ID: UVJ64948.1
• Promoter: COtetR-PIPL12

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CGTCTCGACATATGTCAATTTATCAAGAATTTGTTAATAAATATAG
• 3′ sequencing primer: CGTCTCGGTCTTTAGTTATTCCTATTCTGCACG

Resource Information

• Supplemental Documents:
  • copy-of-ppipl12-fncas12a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry