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pcDNA3-myc3-CUL4A
(Plasmid #19951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CUL4A (cullin 4A)
  • Alt name
    CUL4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2982
  • GenBank ID
    NM_001008895
  • Entrez Gene
    CUL4A
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CUL4A insert in this plasmid contains a K644R substitution when compared to GenBank reference sequence NM_001008895.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-CUL4A was a gift from Yue Xiong (Addgene plasmid # 19951 ; http://n2t.net/addgene:19951 ; RRID:Addgene_19951)

  • For your References section:

    NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Mol Cell. 2002 Dec . 10(6):1511-8. 10.1016/S1097-2765(02)00783-9 PubMed 12504025
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