pcDNA3-HA-CUL1 delta N53
(Plasmid
#19950)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19950 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-HA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecullin 1 delta N53
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Alt nameCUL1
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SpeciesH. sapiens (human)
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Insert Size (bp)2260
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Mutationdeletion of 53 residues from the NH2-terminus(delta N53)
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GenBank IDNP_003583
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Entrez GeneCUL1
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-CUL1 delta N53 was a gift from Yue Xiong (Addgene plasmid # 19950 ; http://n2t.net/addgene:19950 ; RRID:Addgene_19950) -
For your References section:
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Mol Cell. 2002 Dec . 10(6):1511-8. 10.1016/S1097-2765(02)00783-9 PubMed 12504025
Map uploaded by the depositor.
