pcDNA3-myc-Skp2
(Plasmid #19947)

• Depositing Lab: Yue Xiong
• Publication: Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S-phase kinase-associated protein 2 (p45)
• Alt name: Skp2
• Alt name: p45
• Species: H. sapiens (human)
• Insert Size (bp): 1305
• GenBank ID:
• Entrez Gene: SKP2 (a.k.a. FBL1, FBXL1, FLB1, p45)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the 436 amino acid Skp2 insert in this plasmid does not exactly match GenBank reference sequences for Skp2. The Skp2 insert was PCR amplified from a HeLa cell library and appears to be a combination of the first 12 amino acids of reference sequence AAC50242.1 (containing a H2D mutation due to the primer used for cloning) followed by amino acids 1-424 of NP_005974.2, with a F343I mutation (numbering according to 436aa Skp2).