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Addgene

pCMV-BpBBS
(Plasmid #199468)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone size w/o insert (bp) 3970
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BpBBS
  • Species
    Boana punctata (Polka-dot tree frog)
  • Insert Size (bp)
    1227

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BpBBS was a gift from Vladislav Verkhusha (Addgene plasmid # 199468 ; http://n2t.net/addgene:199468 ; RRID:Addgene_199468)

  • For your References section:

    Structural and Functional Characterization of a Biliverdin-Binding Near-Infrared Fluorescent Protein From the Serpin Superfamily. Manoilov KY, Ghosh A, Almo SC, Verkhusha VV. J Mol Biol. 2022 Jan 30;434(2):167359. doi: 10.1016/j.jmb.2021.167359. Epub 2021 Nov 16. 10.1016/j.jmb.2021.167359 PubMed 34798132
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