pBAD-BpBBS
(Plasmid
#199467)
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PurposeArabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD/His-B
- Backbone size w/o insert (bp) 3955
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBpBBS
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SpeciesBoana punctata (Polka-dot tree frog)
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Insert Size (bp)1244
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-BpBBS was a gift from Vladislav Verkhusha (Addgene plasmid # 199467 ; http://n2t.net/addgene:199467 ; RRID:Addgene_199467) -
For your References section:
Structural and Functional Characterization of a Biliverdin-Binding Near-Infrared Fluorescent Protein From the Serpin Superfamily. Manoilov KY, Ghosh A, Almo SC, Verkhusha VV. J Mol Biol. 2022 Jan 30;434(2):167359. doi: 10.1016/j.jmb.2021.167359. Epub 2021 Nov 16. 10.1016/j.jmb.2021.167359 PubMed 34798132
