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Addgene

pBAD-degFusionRed
(Plasmid #199463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/His-B
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    degFusionRed
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-degFusionRed was a gift from Vladislav Verkhusha (Addgene plasmid # 199463 ; http://n2t.net/addgene:199463 ; RRID:Addgene_199463)

  • For your References section:

    Photodegradable by Yellow-Orange Light degFusionRed Optogenetic Module with Autocatalytically Formed Chromophore. Chernov KG, Manoilov KY, Oliinyk OS, Shcherbakova DM, Verkhusha VV. Int J Mol Sci. 2023 Mar 30;24(7):6526. doi: 10.3390/ijms24076526. 10.3390/ijms24076526 PubMed 37047499
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