pGEX-Skp2
(Plasmid #19946)

• Depositing Lab: Yue Xiong
• Publication: Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-49.

Backbone

• Vector backbone: pGEX-KG
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 5006
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S-phase kinase-associated protein 2 (p45)
• Alt name: Skp2
• Alt name: p45
• Species: H. sapiens (human)
• Insert Size (bp): 1305
• GenBank ID: NM_005983
• Entrez Gene: SKP2 (a.k.a. FBL1, FBXL1, FLB1, p45)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13
• 3′ sequencing primer: M13

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry