pCA17-CRISPR-Tag (11XTS1) for dSpCas9
(Plasmid
#199448)
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PurposeCRISPR-Tag sequence that can be efficiently bound by dSpCas9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMV
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRISPR-Tag
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA17-CRISPR-Tag (11XTS1) for dSpCas9 was a gift from Baohui Chen (Addgene plasmid # 199448 ; http://n2t.net/addgene:199448 ; RRID:Addgene_199448) -
For your References section:
Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions. Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B. J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. 10.1128/jvi.00920-22 PubMed 36453882