pCA13-pHR-SFFV-HaloTag-FBL
(Plasmid
#199444)
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PurposeExpression of HaloTag-FBL in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 199444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFBL
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SpeciesH. sapiens (human)
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Entrez GeneFBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
- Promoter SFFV
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Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCA13-pHR-SFFV-HaloTag-FBL was a gift from Baohui Chen (Addgene plasmid # 199444 ; http://n2t.net/addgene:199444 ; RRID:Addgene_199444) -
For your References section:
Real-time imaging of RNA polymerase I activity in living human cells. Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. 10.1083/jcb.202202110 PubMed 36282216