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Addgene

pCA12-pHR-SFFV-BFP-B23
(Plasmid #199443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199443 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B23
  • Species
    H. sapiens (human)
  • Entrez Gene
    NPM1 (a.k.a. B23, NPM)
  • Promoter SFFV
  • Tag / Fusion Protein
    • TagBFP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA12-pHR-SFFV-BFP-B23 was a gift from Baohui Chen (Addgene plasmid # 199443 ; http://n2t.net/addgene:199443 ; RRID:Addgene_199443)

  • For your References section:

    Real-time imaging of RNA polymerase I activity in living human cells. Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z, Zou W, Chen B. J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 10.1083/jcb.202202110. Epub 2022 Oct 25. 10.1083/jcb.202202110 PubMed 36282216
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