pHR-iCasp9
(Plasmid #199441)

• Purpose: Express inducible Caspase9 in mammalian cells
• Depositing Lab: Chao Tang
• Publication: Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. doi: 10.1038/s41419-021-04468-z.

Backbone

• Vector backbone: pHR-mCherry
• Backbone size w/o insert (bp): 9976
• Total vector size (bp): 11242
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral
• Selectable markers: mCherry Fluorescence

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: inducible Caspase9
• Species: H. sapiens (human)
• Entrez Gene: CASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)
• Tags / Fusion Proteins:
  • mCherry
  • DmrB (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: ATGGCTTCTAGAGGAGTGCA
• 3′ sequencing primer: CGCGGATCCCGCTGATGTTTTAAAGAAAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a S113R mutation in the DmrB-iCasp9-mCherry insert. This mutation is not known to affect plasmid function.